TNS - exam #3 study guide Exam Questions and Answers what recognize and cut specific double-stranded DNA? - Correct Answers ✅restriction enzymes what do restriction enzymes do? - Correct Answers ✅cut double stranded DNA what are the names of the sites that restriction enzymes cut the double stranded DNA at? - Correct Answers ✅restriction sites what does PCR stand for? - Correct Answers ✅polymerase chain reaction what uses controlled heating/cooling in the presence of DNA synthesizing enzymes to exponentially amplify a small DNA segment? - Correct Answers ✅PCR what does PCR technique do? - Correct Answers ✅uses controlled heating/cooling in the presence of DNA synthesizing enzymes to exponentially amplify a small DNA segment what are single stranded oligodendrocytes that are essential for PCR - Correct Answers ✅primers 1 / 3
TNS - exam #3 study guide Exam Questions and Answers what are primers - Correct Answers ✅single stranded oligodendrocytes during PCR, what specifies what fragment will be amplified by binding the template? - Correct Answers ✅primers what are 2 common vectors?` - Correct Answers ✅1.plasmid
- phages
what does the following describe: once DNA is isolated
(restriction enzymes/ PCR) > have to store the fragment into a vector for future use / amplication? - Correct Answers ✅cloning DNA what is the process of cloning DNA? - Correct Answers ✅isolating DNA (through restriction enzyme/ PCR) and stores the fragment in vector (for future use or amplification)
what does the following process describe: isolating a piece of
DNA and ligating it into a storage vector, producing enough copies of vector for experiment? - Correct Answers ✅molecular cloning 2 / 3
TNS - exam #3 study guide Exam Questions and Answers what is the process of molecular cloning? - Correct Answers ✅isolating a piece of DNA > ligating it into a storage vector > producing enough copies of vector for an experiment what are naturally occurring circles of DNA that act independently, replicating accessory chromosomes in bacteria? - Correct Answers ✅plasmid vectors what are plasmid vectors? - Correct Answers ✅circles of DNA that act independently, replicating accessory chromosomes in bacteria what is a nucleic acid storage system that can hold and isolate DNA sequence of interest? - Correct Answers ✅DNA vector what is a DNA vector? - Correct Answers ✅nucleic acid storage system that can hold an isolated DNA sequence of interest
compare the following:
plasmid vector = ?DNA vector = ? - Correct Answers ✅plasmid vector = circles of DNA that act independently, replicating accessory DNA in bacteria
- / 3