QUESTIONS AND ANSWERS 100

Microbiology Lab Final MIC 206 EXAM

QUESTIONS AND ANSWERS 100%

SOLVED (Newest 2025) ability to transfer microbes from one place to another without contaminating the original culture, the new medium or the environment - Correct Answers ✅define aseptic technique

• disinfecting workbenches before and after use/washing

hands

• sterilizing media and inoculating instruments
• transfer of microbes without contamination - pinky finger

hold of test tube cap, tilting the test tube to minimize air contaminants, working efficiently to reduce to exposure to air, etc. - Correct Answers ✅list 3 examples of aseptic technique used in lab

• broths are used to grow microbes when fresh cultures or

large numbers are needed

• slants are used to grow and maintain stock cultures -

Correct Answers ✅what are the different uses of agar slants compared to broths

• seconds should be sufficient to get the orange flow -

Correct Answers ✅how many seconds should you sterilize your inoculation loop in the bacteria-incinerator?the purpose of a streak plate is to isolate individual colonies (species) from a mixed sample - Correct Answers ✅what is the purpose of a streak plate 1 / 4

Microbiology Lab Final MIC 206 EXAM

QUESTIONS AND ANSWERS 100%

SOLVED (Newest 2025) a sterile swab is used to inoculate a lawn culture so that growth is even, consistent and heavy - Correct Answers ✅what inoculating device is used for a lawn culture keeping the cap suspended in the pinky finger helps prevent contamination of the original culture and the new medium - Correct Answers ✅when making transfers from a test tube, why is it important to never put the cap down?oil is placed between the slide and the lens in order to reduce the refractive index allowing all the light passing through the specimens to be "caught" - Correct Answers ✅what is oil immersion?composed of several objective lens and an ocular lens.together they magnify the object from low power (4x) to high power (40x) - Correct Answers ✅what are compound light microscopes?condenser lens - Correct Answers ✅what is the lens below the stage on the microscope that focuses light through specimen objective lens - Correct Answers ✅lens that magnifies image (4x, 10x, 40x, or 100x) 2 / 4

Microbiology Lab Final MIC 206 EXAM

QUESTIONS AND ANSWERS 100%

SOLVED (Newest 2025) ocular lens - Correct Answers ✅Eyepiece that contains 10x lens mechanical stage/ stage control - Correct Answers ✅Moves specimen left / right and backward / forward fine adjustment - Correct Answers ✅Knob used to sharpen image of specimen coarse adjustment - Correct Answers ✅Knob used at low magnification (4x) to raise or lower the stage in order to find specimen 4x, 10, 40, 100x - Correct Answers ✅magnifications of objective lens You can tell the whether the bacteria is Gram + or Gram -, you can see the cell shape and size, and the arrangement. - Correct Answers ✅What information can we obtain about the bacteria by doing a Gram stain?A smear is a thin layer (one cell thick) of bacterial cells attached to a microscope slide. - Correct Answers ✅define smear 3 / 4

Microbiology Lab Final MIC 206 EXAM

QUESTIONS AND ANSWERS 100%

SOLVED (Newest 2025) crystal violet, grams iodine , alcohol decolorizer, safranin - Correct Answers ✅name all of the chemical components in a gram stain crystal violet - Correct Answers ✅a chemical used in a

gram stain: Adheres to the peptidoglycan of both bacterial

cell wall types grams iodine - Correct Answers ✅a chemical used in a

gram stain: Contains potassium iodide which acts as a

mordant to help strengthen the bond between crystal violet and peptidoglycan by making the molecule larger, thus preventing crystal violet from washing off alcohol decolorizer - Correct Answers ✅a chemical used in

a gram stain: used to wash away crystal violet from both cell

walls. However, in Gram + more crystal violet is absorbed in the thick layers of peptidoglycan retaining the purple color. In Gram - all crystal violet is washed away.safranin - Correct Answers ✅a chemical used in a gram

stain: counterstain that adheres to the lipid membranes. In

Gram - this will be the only stain visible. In Gram +, this stain will be present, but it does not over power crystal violet.

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