MIC 206 Final Practical Downtown EXAM
QUESTIONS AND ANSWERS 100%
SOLVED (Newest 2025) methylene blue, crystal violet, safranin are all examples of what kind of stain? - Correct Answers ✅basic stains nigrosin is an example of what kind of stain? - Correct Answers ✅acidic stains simple staining procedure - Correct Answers ✅1. smear preparation
- fixation (heat)
- staining
- Correct Answers ✅gram positive bacteria; because it has more
a special absorbent paper used to dry slides; blotting paper - Correct Answers ✅bibulous paper what objective lens should you always start with? - Correct Answers ✅10x When focusing the ___ objective(s), you only adjust the fine focus adjustment/knob. - Correct Answers ✅40x and 100x cell wall component unique to bacteria, largely responsible for the strength of the cell wall - Correct Answers ✅peptidoglycan Which type of Gram bacteria can withstand a higher internal pressure? why?
peptidoglycan which type of Gram bacteria has a cell membrane? - Correct Answers ✅gram negative bacteria 1 / 3
MIC 206 Final Practical Downtown EXAM
QUESTIONS AND ANSWERS 100%
SOLVED (Newest 2025) First stain applied after heat fixing - Correct Answers ✅primary stain what stain determines the color of Gram positive cells? - Correct Answers ✅primary stain color fixative, almost always Gram's iodine - Correct Answers ✅mordant works by forming a complex molecule with the primary dye so that it is not completely leached out of thick peptidoglycan - Correct Answers ✅mordant removes primary stain from gram negative cells; usually ethanol - Correct Answers ✅decolorizer determines gram negative cells; safranin is an example of this - Correct Answers ✅counter-stain gram stain procedure - Correct Answers ✅1. smear prep & heat fix
- crystal violet (60sec)
- wash with water
- Gram's iodine (60 sec)
- wash with water
- 95% ethanol ( 2-3 sec)
- wash with water
- safranin (60 sec)
- wash with water
- blot dry 2 / 3
MIC 206 Final Practical Downtown EXAM
QUESTIONS AND ANSWERS 100%
SOLVED (Newest 2025) Allows one to identify cell morphology and arrangement - Correct Answers ✅simple staining Allows one to differentiate 90% of bacterial species into two large groups (Gram-positive and Gram- negative) based on the chemical and physical properties of their cell walls (peptidoglycan) - Correct Answers ✅gram staining Unique cell wall which contains mycolic acid - Correct Answers ✅acid fast bacteria composed of fatty acids, waxes, and complex lipids that make up the cell wall; mycobacteria contains this - Correct Answers ✅mycolic acid acid-fast stain is formerly known as - Correct Answers ✅Ziel-Neelsen stain acid-fast stain procedure - Correct Answers ✅1. smear prep & heat fix
- carbolfuchsion over steam (8min)
- wash with water
- acid alcohol (1-2sec)
- wash with water
- methylene blue (60sec)
- wash with water
- blot dry
- / 3
acid-fast stain