MLT-MLS Hematology 2019-2020 UAMS exam 1 review 100% Complete Best Solution % of basophils - Correct Answer 0-2%
% of eosinophils - Correct Answer 1-3%
% of lymphocytes - Correct Answer 20-40%
% of monocytes - Correct Answer 2-11%
Appearance of water artifacts on the smear - Correct Answer dry slides as quickly as possible or fix slides with methanol
banded neutrophil - Correct Answer contains pink cytoplasm with specific granules and a nucleus with coarse chromatin that is deeply indented more than 50% of the width of the nucleus
Basophils morphology - Correct Answer -most uncommon of granulocytes: have blue- purplish granules, and usually hidden nucleus -release histamine
Buffer - Correct Answer Controls the pH of the staining
Can NRBCs be found on a newborn blood smear? - Correct Answer yes
Do RBC's have granules? - Correct Answer Nope
Do RBCs become larger or smaller as they mature? - Correct Answer Smaller
Effects on Manual WBC counts: Calculating wrong - Correct Answer increase or
decrease
Effects on Manual WBC counts: Counting the same square twice - Correct Answer
increase or decrease
Effects on Manual WBC counts: Elevated RBC count - Correct Answer not affected
Effects on Manual WBC counts: Mixing the dilutions before loading chambers - Correct Answer not affected
Effects on Manual WBC counts: Not cleaning excess blood off the outside of the pipette tip - Correct Answer increase
Effects on Manual WBC counts: Not drying out the methanol from the chamber before loading - Correct Answer Decrease 1 / 2
Effects on Manual WBC counts: Not letting cells settle on the chamber before counting
- Correct Answer decrease
Effects on Manual WBC counts: Not mixing the dilution before loading the chambers - Correct Answer Increase or decrease
Effects on Manual WBC counts: Pulling blood out of the pipet when wiping the excess blood from the tip - Correct Answer Decrease
Eosin - Correct Answer Acidic and stains basic and eosinophilic components such as hemoglobin and eosinophilic granules
Eosinophil morphology - Correct Answer elongated band-shaped, bi-lobed or tri-lobed nucleus cytoplasm has red/red-orange staining granules
feline: numerous small rod-shaped granules
canine: varying size granules in same cell
-seen during parasitic infection
Extravascular - Correct Answer outside the blood vascular system
Hematopoiesis - Correct Answer formation and development of blood cells occurring primarily in the bone marrow and peripheral lymphatic tissues
How are WBC distinguished from platelets? - Correct Answer size
How can you tell the difference between a monocyte and a banded neutrophil? - Correct Answer Banded neutrophil has pinker cytoplasm
How can you tell the difference between a Myeloblast and a lympyocyte? - Correct Answer Myeloblast is much bigger lymphocyte nucleus is much darker
How long do RBCs live? - Correct Answer 120 days
If RCBs on a slide are too blue, what is needed to be done? - Correct Answer Decrease the staining time
If someone one is able to get the smear focused with 10X and 40X but has a problem on 100X, what could be the problem? - Correct Answer Verify the slide orientation (make sure the stained slide is up) Ensure oil is added
Intramedullary Hemolysis - Correct Answer cell destruction inside the bone marrow
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