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MLS (Medical Laboratory Scientist) ASCP Exam Newest 2025 with accurate and verified questions covering clinical chemistry, hematology, microbiology, immunohematology, molecular diagnostics, laboratory operations, and quality control. Designed to prepare candidates for ASCP certification with up-to-date clinical and procedural content.
Which of the following Rh antigens is found the highest
frequency in the Caucasian population:
- C
- E
- c
- e - ANSWER-D. e
The accuracy of an immunoassay is its ability to discriminate between results that are true positives and results that are true negatives. Two parameters of test accuracy are specificity and sensitivity. Which of these statements apply to an immunoassay with high specificity, but low sensitivity? - ANSWER- 1 / 4
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Accurately identifies the absence of disease and has few false- positives
A test with high specificity accurately detects the absence of disease. The more specific a test is, the fewer false-positive results will occur. A test with high sensitivity accurately identifies the presence of disease. The more sensitive a test, the fewer false-negative results it produces. In the case stated in this question, the immunoassay has high specificity, so it has few false-positives and will accurately detect those individuals who do not have the disease or condition that is being tested for.However, the test has low sensitivity, so it may not identify all individuals who actually have the disease; it may produce many false-negative results.
Distinguishing characteristics of Micrococcus luteus: -
ANSWER-Resistance to furazolidone is one of the key characteristics by which members of the genus Micrococcus (resistant) can be separated from members of the genus Staphylococcus (susceptible). Micrococcus species also are susceptible to novobiocin and to bacitracin, although the zone of inhibition to the "A" disk is often small (as shown in this photograph).
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The sickling phenomenon observed in erythrocytes containing hemoglobin S may be induced by which of the following? - ANSWER-Reducing agents actually induce the sickling phenomenon in RBCs when they contain hemoglobin S.
If an autologous blood donor weighs 35 kg, how much blood can be collected for later transfusion? - ANSWER-315 mL
Volume to draw = (Donor's weight in kilograms / 50 kg (minimum weight requirement) x 450 mL (volume of unit to be donated)
In this case, Volume to draw = (35 kg / 50 kg) x 450 mL = 315 mL
Which adipokine is decreased in obesity, is anti-inflammatory, and increases insulin sensitivity? - ANSWER-Adiponectin is a protective cytokine. This cytokine is anti-inflammatory and increases insulin sensitivity. Adiponectin is usually decreased in obesity.
Distinguishing characteristics of Mycobacterium fortuitum: -
ANSWER-Mycobacterium fortuitum is positive for iron uptake, 3 / 4
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nitrate, arysulfatase, and growth on MacConkey agar without crystal violet in five days.
CD5 antigen is normally found on which of the following lymphocyte populations? - ANSWER-Mature T cells
Define: Congestive heart failure - ANSWER-A left ventricular
dysfunction resulting from aging, hypertension, atherosclerosis or muscle damage from an AMI or repeated AMIs.
Define infarction: - ANSWER-An area of tissue death that
occurs due to lack of oxygen.
Define: Ischemia - ANSWER-An inadequate blood supply that
decreases availability of oxygen.
Define: Angina - ANSWER-Chest pain caused by inadequate
supply of oxygen to heart myocardium.
Which of the following antigen groups is closely related to the ABO antigens on the red cell membrane? - ANSWER-I and i antigens are related biochemically and spatially to the ABH and Lewis antigens on the red cell membrane. Anti-I is usually an
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