MLS 2211 Unit 1 Chem WSU with Verified

MLS 2211 Unit 1 Chem WSU with Verified Solutions

Dilution The amount of sample divided by the total amount of solution You have 1 mL of sample and 4 mL of H2O

• How many parts do you have?
• Whats the ratio?
• What is the dilution factor?
• What is the concentration of the sample after its diluted?
• (4+1) = 5 parts

- 1:5

• The dultion factor is 5

- 0.20

The dilution factor is the deminator 5 You have 5 mL of sample and 5 mL of H2O

• how many parts do you have?
• Whats the ratio?
• What is the dilution factor? 1 / 3

• What is the concentration of the sample after its diluted?
• 10 parts

- 1:2

• 2
• 0.5

You have 5 mL of sample and 10 mL of H2O

• How many parts do you have?
• Whats the ratio
• What is the dilution factor?
• What is the concentration of the sample after its been diluted?
• 15 parts

- 1:3

• 3

- 0.3333

serial dilution A stepwise dilution of a substance in solution When would we use serial dilution in the lab?when we want to access antibody concentration/strength aka titers.

How would you prepare a 1:24 dilution? 2 / 3

• 1 part sample
• 23 part H2O
• add the component to an actual amount ex sample has 5 uL and the H2O has 115uL
• total volume = 120uL

-120/5 = 24

therefore 1:24

What if you have too much of something?dilution Dilution (The golden clinical chemistry equation)

C1V1=C2V2

c1 = v1=

• initial concentration
• initial volume

c2= v2=

• concentration of the diluted solution
• volume of the diluted solution
• mL of a 50 mg/dL sample is diluted up to a final volume of 15 mL. What is the final

concentration?

3.333 mg/dl

• / 3