FRSC 2030 Final EXAM QUESTIONS AND ANSWERS 100% SOLVED (Newest 2025) Forensic scientist should - Correct Answers ✅put physical evidence into context, not prove innocence or guilt Purpose of Presumptive Test - Correct Answers ✅help provide initial context at crime scenes as to where biological stains are, what type biological stain is present, and the patterns of the biological stains
- categories of presumptive tests - Correct Answers
✅colour tests, luminescence tests, and antibody precipitin tests Kastle-Meyer Test - Correct Answers ✅phenolphthalein reacts with the blood's hemoglobin in the presence of hydrogen peroxide and is oxidized from its colorless form to a bright/deep pink color, not human blood specific Hemastix - Correct Answers ✅fast and easy, a strip that has been coated with tetramethylbenzidine (TMB) and will produce a green color with the presence of hemoglobin, not specific to human blood Luminol and bluestar - Correct Answers ✅react with oxidizing agents, such as the heme in blood, to emit light, or luminescence 1 / 4
FRSC 2030 Final EXAM QUESTIONS AND ANSWERS 100% SOLVED (Newest 2025) Advantage of luminol/bluestar - Correct Answers ✅finding minute traces of blood, including after a crime scene has been cleaned and can tell if body was moved Drawback of luminol/bluestar - Correct Answers ✅reaction is not specific to blood, and other oxidizing agents like bleach, some metals, and plant material can also cause luminescence with luminol Precipitin Tests - Correct Answers ✅serological tests to detect the presence of a specific antigen, such as human specific blood antigens Hematrace - Correct Answers ✅uses principles of the precipitin test to detect human heme in hemoglobin antigens, reacts with antihuman antibodies labeled with pink dye, pink line formed when antigens/antibodies complex Tests for Semen - Correct Answers ✅large percentage of cases are sexual assault, microscopic examination of spermatozoa is performed along with presumptive and confirmation tests Presumptive and confirmation semen test - Correct Answers ✅presumptive- acid phosphatase test (colour) confirmation- prostate specific antigen test (human) 2 / 4
FRSC 2030 Final EXAM QUESTIONS AND ANSWERS 100% SOLVED (Newest 2025) Acid phosphatase test - Correct Answers ✅high levels in human semen, changes colour when it detects mono- phenolic phosphoric acid, can take a a while to react p30 test - Correct Answers ✅human semen contains high levels of p30 (prostate specific antigen),protein that is specific to semen with few exceptions, test very sensitive Presumptive tests for saliva - Correct Answers ✅Phadebas test for alpha-amylase turns blue when present Other bodily fluids that have acid phosphatase - Correct Answers ✅semen and vaginal secretions Why are antibody tests important in identifying bodily fluids beyond just colour tests? - Correct Answers ✅they are often more specific, and less likely to give false positives based on cross-reactivity with other substances Somatic cells - Correct Answers ✅can identify an individual from dna bc each cell has 2 copies of entire genome 2n Gametes (sperm or egg) - Correct Answers ✅haploid (one copy of each of the 23 chromosomes), 1n 3 / 4
FRSC 2030 Final EXAM QUESTIONS AND ANSWERS 100% SOLVED (Newest 2025) A single sperm would have - Correct Answers ✅1n copies of genome Coding part of DNA - Correct Answers ✅DNA that encodes for proteins (approx 5% of genome) locus - Correct Answers ✅specific section of DNA, more sections adding power to ID individuals Mendelian Inheritance - Correct Answers ✅getting half your DNA from each of your parents Mitochondrial DNA (mtDNA) - Correct Answers ✅small, several hundred copies in a given cell, maternally inherited Advantages of mtDNA - Correct Answers ✅many more copies per cell than nDNA, degraded evidence will more likely provide a mtDNA profile Disadvantages to mtDNA - Correct Answers ✅ability to distinguish between individuals is much lower for mtDNA bc you can only trace maternal lineage What is the ploidy of human nDNA? - Correct Answers ✅Diploid, copy from mom and a copy from dad
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