CHEM Lab 8 Report Organic Chemistry Dayna Cox
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Background:
Lab 8 The goal of Lab 8 is to extract Limonene using steam distillation (separating
compound with different boiling points). Two techniques will be used: steam
distillation and liquid-liquid extraction. Steam distillation is purifying a liquid in steam conditions due to the liquid being difficult to purify in a normal temperature state. Liquid-liquid extraction will then be performed to separate the organic and aqueous layer.
Key Experimental Details:
Dried orange zest is provided. 5.01g of orange zest is obtained and put into a 100mL round bottom flask along with 50mL of DI water to soak for 10 minutes. After soaking is complete add 2 drops of antifoam agent and a couple of boiling chips into flask (3 boiling chips were added). The distillation flask should be wrapped in aluminm foil to conserve heat (the top and bottom should be covered). A hot plate should be on heat to boil the water with orange zest. Fill receiving flask with 25mL of water and mark the level with sharpie, this is only so we can measure 25mL in the flask (this is in separate flask). After marking 25mL of water on the flask, pour out the water. Assembling of the steam distillation is to be set up and distillate should start collecting in receiver flask in about 30 minutes after heat is turned on.25mL of distillate should collect into the receiver, this can be observed by the sharpie line of 25mL. This will determine the distillation of limonene being complete. Transfer distillate to separatory funnel. Gently mix the two layers and allow them to separate. Isolate the organic layer in Erlenmeyer flask and dry using anhydrous sodium sulfate. Decant and store into organic layer in test tube; seal with cork and parafilm, then store in drawer.
Next week:
The limonene sample is to be analyzed in Ethyl Acetate. This is to be compared to standard sample of Limonene in Ethyl Acetate (its provided). Comparing the results of the two GC’s runs you should be able to calculate the amount of Limonene present in your distillate in mg/mL.
Results:
CompoundVolume Boiling Point Distillate collected 25mL
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