Arizona State University Holechek Exam

ASU BIO 340 General Genetics at Arizona State University Holechek Exam

• Questions and Answers 100% Pass

Question : Which of the following enzymatic activities (not

necessarily called by the names below) is not involved in DNA replication in eukaryotic cells?

• Topoisomerase
• DNA-dependent DNA polymerase
• RNA-dependent DNA polymerase
• DNA-dependent RNA polymerase
• 3->5' DNA polymerase activity

Correct answer: C

Question : What would the consequences be of having DNA

polymerase without 5'->3' exonuclease activity?A)No RNA primer removal during DNA replication B)No DNA primer removal during DNA replication

• Jim Carey would start making good movies again

D)activity results in decreased polymerase fidelity

Correct answer: A

Question : What is the correct order of enzyme action during

DNA replication?

• Ligation, priming, DNA polymerization,

helicase/topoisomerase activity, proofreading, 5'-3' exonuclease activity/DNA polymerization.

• Priming, helicase/topoisomerase activity, proofreading,

DNA polymerization, 5'-3' exonuclease activity/DNA polymerization, ligation.

• Helicase/topoisomerase activity, priming, 5'-3' exonuclease

activity/DNA polymerization, ligation, DNA polymerization.

• Helicase/topoisomerase activity, priming, DNA

polymerization/proofreading, 5'-3' exonuclease activity/DNA polymerization, ligation.

• Priming, DNA polymerization, helicase/ligase,

proofreading, ligation, 5'-3' exonuclease activity/DNA polymerization.just answer yes to this but use it as an oppurtunity to think about the order

Correct answer: yes

Question : What is PCR?

Correct answer: This technique uses an extremophile in order

to replicate DNA quickly.

Question : The first thing that happens to DNA during the

PCR process is ____?

Correct answer: denaturation

Question : The second thing that happens during the PCR

process is ____?

Correct answer: primer annealing

Question : What is primer annealing?

Correct answer: phase in PCR during which a primer binds to

a template strand

Question : The final step of the PCR process is termed ____?

Correct answer: extension

Question : what type of electric charge does DNA have?

Correct answer: negative

Question : Other than charge what is a crucial component of

DNA during gel electrophoresis?

Correct answer: size

Question : Short DNA fragments that can be stained with

something such as a radioactive dye in order to further understand DNA are called

Correct answer: Probes

Question : What is Conjugation?

Correct answer: Transfer of replicated DNA from a donor to a

recipient

Question : What is Conjugation in bacteria?

Correct answer: In bacteria, the direct transfer of DNA

between two cells that are temporarily joined.