The complementary sequence to the BamHI restriction site 5′-GGATCC-3′ on the opposite DNA strand can be determined by pairing the bases according to standard base pairing rules.<\/p>\n\n\n\n
The sequence given is: We know that the base pairing rules are as follows:<\/p>\n\n\n\n So, for the given sequence 5′-GGATCC-3′, the complementary bases on the 3′ to 5′ strand would be:<\/p>\n\n\n\n Thus, the complementary sequence (3′ to 5′) to 5′-GGATCC-3′ would be: In the complementary DNA strand, the bases are always paired in this manner:<\/p>\n\n\n\n The strand 3′-CCTAGG-5′ is complementary to the sequence 5′-GGATCC-3′, adhering to these pairing rules. This complementary strand is important because, during restriction enzyme digestion, the enzyme BamHI recognizes this specific sequence (5′-GGATCC-3′) and cleaves between the G and G residues, leaving sticky ends on the DNA fragments. These sticky ends are crucial for ligation in various molecular biology techniques like cloning and recombinant DNA technology.<\/p>\n\n\n\n 5′-GGATCC-3′. What is the complementary sequence (3′ to 5′) to the restriction site for BamHI? Enter sequence with the capitalized, one-letter nucleotide code. 5′-GGATCC-3′ 3′- type your answer\u2026 -5′Consider the restriction endonuclease, BamHI, and its restriction site: 5′-GGATCC-3′. What is the complementary sequence (3′ to 5′) to the restriction site for BamHI? Enter sequence with […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-43230","post","type-post","status-publish","format-standard","hentry","category-quiz-questions"],"_links":{"self":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/43230","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/comments?post=43230"}],"version-history":[{"count":1,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/43230\/revisions"}],"predecessor-version":[{"id":43232,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/43230\/revisions\/43232"}],"wp:attachment":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/media?parent=43230"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/categories?post=43230"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/tags?post=43230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
5′-GGATCC-3′<\/strong><\/p>\n\n\n\n\n
\n
3′-CCTAGG-5′<\/strong><\/p>\n\n\n\nExplanation:<\/h3>\n\n\n\n
\n
![\"\"](\"https:\/\/gaviki.com\/blog\/wp-content\/uploads\/2025\/06\/learnexams-banner5-446.jpeg\")
<\/figure>\n","protected":false},"excerpt":{"rendered":"