{"id":35850,"date":"2025-06-24T17:44:43","date_gmt":"2025-06-24T17:44:43","guid":{"rendered":"https:\/\/gaviki.com\/blog\/?p=35850"},"modified":"2025-06-24T17:44:55","modified_gmt":"2025-06-24T17:44:55","slug":"a-test-tube-containing-a-solution-of-rna-would-turn-blue-if-dische-diphenylamine-test-reagent-is-added","status":"publish","type":"post","link":"https:\/\/gaviki.com\/blog\/a-test-tube-containing-a-solution-of-rna-would-turn-blue-if-dische-diphenylamine-test-reagent-is-added\/","title":{"rendered":"A test tube containing a solution of RNA would turn blue if Dische diphenylamine test reagent is added"},"content":{"rendered":"\n<p>A test tube containing a solution of RNA would turn blue if Dische diphenylamine test reagent is added. True False<\/p>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">The Correct Answer and Explanation is:<\/mark><\/strong><\/p>\n\n\n\n<p><strong>Correct Answer: False<\/strong><\/p>\n\n\n\n<p><strong>Explanation:<\/strong><\/p>\n\n\n\n<p>The Dische diphenylamine test is a chemical test specifically used to detect <strong>deoxyribose<\/strong>, the sugar found in <strong>DNA<\/strong>. It is not intended to detect <strong>ribose<\/strong>, the sugar found in <strong>RNA<\/strong>. The test works by reacting with the deoxyribose sugar in acidic conditions to produce a <strong>blue-colored complex<\/strong>. Therefore, only DNA-containing solutions give a blue color when treated with Dische diphenylamine reagent.<\/p>\n\n\n\n<p>In contrast, RNA contains <strong>ribose<\/strong> rather than deoxyribose. The structural difference between the two sugars lies in the presence of a hydroxyl group on the second carbon in ribose, which is absent in deoxyribose. Because of this difference, ribose does <strong>not<\/strong> react with the diphenylamine reagent in the same way. As a result, when the Dische test is applied to an RNA solution, <strong>no blue coloration<\/strong> develops. There might be a slight color change depending on impurities or experimental conditions, but it is not the characteristic blue that indicates the presence of DNA.<\/p>\n\n\n\n<p>The Dische test is useful in distinguishing between DNA and RNA in biochemical experiments. In situations where it is necessary to confirm the presence of DNA in a sample, the development of a blue color upon adding Dische diphenylamine reagent is a positive indication. Since RNA does not give this result, the test also helps confirm the <strong>absence of DNA<\/strong> when no blue color is observed.<\/p>\n\n\n\n<p>To summarize, a test tube containing a solution of RNA <strong>would not<\/strong> turn blue when Dische diphenylamine reagent is added. Therefore, the statement is <strong>false<\/strong>, because the test is specific for DNA due to its reaction with deoxyribose, not with ribose found in RNA.<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"852\" height=\"1024\" src=\"https:\/\/gaviki.com\/blog\/wp-content\/uploads\/2025\/06\/learnexams-banner8-705.jpeg\" alt=\"\" class=\"wp-image-35858\" srcset=\"https:\/\/gaviki.com\/blog\/wp-content\/uploads\/2025\/06\/learnexams-banner8-705.jpeg 852w, https:\/\/gaviki.com\/blog\/wp-content\/uploads\/2025\/06\/learnexams-banner8-705-250x300.jpeg 250w, https:\/\/gaviki.com\/blog\/wp-content\/uploads\/2025\/06\/learnexams-banner8-705-768x923.jpeg 768w\" sizes=\"auto, (max-width: 852px) 100vw, 852px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>A test tube containing a solution of RNA would turn blue if Dische diphenylamine test reagent is added. True False The Correct Answer and Explanation is: Correct Answer: False Explanation: The Dische diphenylamine test is a chemical test specifically used to detect deoxyribose, the sugar found in DNA. It is not intended to detect ribose, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-35850","post","type-post","status-publish","format-standard","hentry","category-quiz-questions"],"_links":{"self":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/35850","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/comments?post=35850"}],"version-history":[{"count":1,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/35850\/revisions"}],"predecessor-version":[{"id":35859,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/35850\/revisions\/35859"}],"wp:attachment":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/media?parent=35850"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/categories?post=35850"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/tags?post=35850"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}