{"id":34197,"date":"2025-06-23T14:10:44","date_gmt":"2025-06-23T14:10:44","guid":{"rendered":"https:\/\/gaviki.com\/blog\/?p=34197"},"modified":"2025-06-23T14:10:45","modified_gmt":"2025-06-23T14:10:45","slug":"calculate-the-specific-activity-fold-purification-and-the-yield-for-each-step-as-compared-to-the-initial-starting-material-and-fill-in-the-values-in-the-appropriate-columns","status":"publish","type":"post","link":"https:\/\/gaviki.com\/blog\/calculate-the-specific-activity-fold-purification-and-the-yield-for-each-step-as-compared-to-the-initial-starting-material-and-fill-in-the-values-in-the-appropriate-columns\/","title":{"rendered":"Calculate the specific activity, fold-purification, and the % yield for each step as compared to the initial starting material and fill in the values in the appropriate columns."},"content":{"rendered":"\n

Calculate the specific activity, fold-purification, and the % yield for each step as compared to the initial starting material and fill in the values in the appropriate columns. Total Recovery Protein (\u00ce\u00bcg) Specific Activity Fold-Purification % Yield Youm Hep Enzyme Units Initial Starting Material 470 7949<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

The Correct Answer and Explanation is:<\/mark><\/strong><\/p>\n\n\n\n

Here is the completed table with all required values filled in, followed by a detailed explanation.<\/p>\n\n\n\n

Step<\/th>Total Recovery Protein (\u00b5g)<\/th>Enzyme Units<\/th>Specific Activity (U\/\u00b5g)<\/th>Fold-Purification<\/th>% Yield<\/th><\/tr><\/thead>
Initial Starting Material<\/td>470<\/td>7949<\/td>16.91<\/td>1.00<\/td>100.00<\/td><\/tr>
40% (NH\u2084)\u2082SO\u2084 Purification<\/td>181<\/td>3991<\/td>22.05<\/td>1.30<\/td>50.22<\/td><\/tr>
DEAE Flow Through, pH 7.0<\/td>67.6<\/td>3787<\/td>56.02<\/td>3.31<\/td>47.63<\/td><\/tr>
Affinity Chromatography<\/td>3.8<\/td>2949<\/td>776.05<\/td>45.90<\/td>37.09<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Explanation:<\/h3>\n\n\n\n

To complete the table, three key parameters are computed at each purification step: specific activity<\/strong>, fold-purification<\/strong>, and percent yield<\/strong>, all relative to the initial material.<\/p>\n\n\n\n

Specific activity<\/strong> quantifies enzyme purity. It is calculated by dividing enzyme units by protein mass in micrograms. For example, for the initial step: Specific activity = 7949 U \/ 470 \u00b5g = 16.91 U\/\u00b5g.<\/p>\n\n\n\n

Fold-purification<\/strong> compares each step\u2019s specific activity to that of the crude extract. It shows how much purer the enzyme has become. At the affinity chromatography step, we get: Fold-purification = 776.05 \/ 16.91 = 45.90.<\/p>\n\n\n\n

Percent yield<\/strong> measures how much enzyme activity remains after each step, relative to the initial activity: % Yield = (Enzyme Units at step \/ Initial Enzyme Units) \u00d7 100. At the final step: (2949 \/ 7949) \u00d7 100 = 37.09%.<\/p>\n\n\n\n

These metrics allow researchers to assess efficiency. High specific activity and fold-purification indicate success in isolating the enzyme of interest, while yield shows how much usable enzyme remains. As seen above, as purity increases, protein quantity decreases \u2014 a common tradeoff in biochemical purification.<\/p>\n\n\n\n

\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"

Calculate the specific activity, fold-purification, and the % yield for each step as compared to the initial starting material and fill in the values in the appropriate columns. Total Recovery Protein (\u00ce\u00bcg) Specific Activity Fold-Purification % Yield Youm Hep Enzyme Units Initial Starting Material 470 7949 The Correct Answer and Explanation is: Here is the […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-34197","post","type-post","status-publish","format-standard","hentry","category-quiz-questions"],"_links":{"self":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/34197","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/comments?post=34197"}],"version-history":[{"count":1,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/34197\/revisions"}],"predecessor-version":[{"id":34209,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/34197\/revisions\/34209"}],"wp:attachment":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/media?parent=34197"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/categories?post=34197"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/tags?post=34197"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}