{"id":20924,"date":"2025-06-14T20:55:10","date_gmt":"2025-06-14T20:55:10","guid":{"rendered":"https:\/\/gaviki.com\/blog\/?p=20924"},"modified":"2025-06-14T20:55:12","modified_gmt":"2025-06-14T20:55:12","slug":"which-of-the-following-laboratory-procedures-is-used-for-culturing-animal-viruses-in-the-laboratory","status":"publish","type":"post","link":"https:\/\/gaviki.com\/blog\/which-of-the-following-laboratory-procedures-is-used-for-culturing-animal-viruses-in-the-laboratory\/","title":{"rendered":"Which of the following laboratory procedures is used for culturing animal viruses in the laboratory"},"content":{"rendered":"\n<p>Which of the following laboratory procedures is used for culturing animal viruses in the laboratory? a. embryonated eggs b. broth cultures c. both cell cultures and embryonated eggs d. synthetic cytoplasm<\/p>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\"><strong>The correct answer and explanation is:<\/strong><\/mark><\/p>\n\n\n\n<p><strong>Correct Answer: c. both cell cultures and embryonated eggs<\/strong><\/p>\n\n\n\n<p><strong>Explanation (300 words):<\/strong><\/p>\n\n\n\n<p>Culturing animal viruses in a laboratory requires living cells because viruses are obligate intracellular parasites \u2014 they cannot replicate outside a living host cell. Two commonly used laboratory methods for culturing animal viruses are <strong>cell cultures<\/strong> and <strong>embryonated eggs<\/strong>.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>1. Cell Cultures:<\/strong><\/h3>\n\n\n\n<p>This is the most widely used method in modern virology. In this procedure, animal cells are grown in a controlled environment in a nutrient medium. These cultured cells can be of different types:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Primary cell cultures<\/strong>: Derived directly from animal tissue and have a limited lifespan.<\/li>\n\n\n\n<li><strong>Diploid cell lines<\/strong>: Can divide a limited number of times.<\/li>\n\n\n\n<li><strong>Continuous cell lines<\/strong>: Transformed cells that can be propagated indefinitely (e.g., HeLa cells).<\/li>\n<\/ul>\n\n\n\n<p>Viruses are introduced to the cultured cells, where they infect, replicate, and often cause observable changes known as <strong>cytopathic effects (CPE)<\/strong>, which help identify the virus.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>2. Embryonated Eggs:<\/strong><\/h3>\n\n\n\n<p>This method has been used since the 1930s and is still valuable, especially in vaccine production (e.g., influenza vaccines). A fertilized chicken egg (usually 7\u201312 days old) contains various compartments (chorioallantoic membrane, amniotic cavity, allantoic cavity, yolk sac), each of which can support the growth of different viruses. The specific site of inoculation depends on the virus being studied. This method is cost-effective and provides a sterile environment for viral growth.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Other Options Explained:<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Broth cultures<\/strong> are used for growing bacteria, not viruses.<\/li>\n\n\n\n<li><strong>Synthetic cytoplasm<\/strong> is not a method for culturing viruses, as it lacks the full machinery needed for viral replication.<\/li>\n<\/ul>\n\n\n\n<p>Thus, <strong>both cell cultures and embryonated eggs<\/strong> are recognized and effective methods for cultivating animal viruses in the laboratory, making option <strong>(c)<\/strong> the correct answer.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Which of the following laboratory procedures is used for culturing animal viruses in the laboratory? a. embryonated eggs b. broth cultures c. both cell cultures and embryonated eggs d. synthetic cytoplasm The correct answer and explanation is: Correct Answer: c. both cell cultures and embryonated eggs Explanation (300 words): Culturing animal viruses in a laboratory [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-20924","post","type-post","status-publish","format-standard","hentry","category-quiz-questions"],"_links":{"self":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/20924","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/comments?post=20924"}],"version-history":[{"count":1,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/20924\/revisions"}],"predecessor-version":[{"id":20925,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/posts\/20924\/revisions\/20925"}],"wp:attachment":[{"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/media?parent=20924"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/categories?post=20924"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gaviki.com\/blog\/wp-json\/wp\/v2\/tags?post=20924"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}